KMID : 0903519880310030219
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology 1988 Volume.31 No. 3 p.219 ~ p.225
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Cloning of Reverse Transcriptase Gene of Avian Sarcoma Virus
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Guntaka, R V
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Abstract
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Reverse transcriptase gene of Avian sarcoma virus(ASV) was cloned with a thermoinducible expression vector, pPL-lambda. E. coli N4830 which carries temperature sensitive cl857 la mbda repressor, was transformed with this pPL-pol plasmid DNA. The RNA transcribed by those tranoformants was isolated and analyzed. It was shown that the inserted reverse transcriptase gene of ASV was transcribed at high-level when cells were grown at high temperature.
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